Develop Bioconductor packages with docker container

Readings links to read: use container I am following the last link. pull the container docker pull bioconductor/bioconductor_full:devel docker images REPOSITORY TAG IMAGE ID CREATED SIZE bioconductor/bioconductor_full devel ae3ec2be7376 3 hours ago 5.7GB seuratv3 latest 9b358ab1fd63 2 days ago 2.76GB It is 5.7G in size. start the Rstuido from the image. I have another Rstudio instance using port 8787, let me use a different one (e.

Calculate scATACseq TSS enrichment score

TSS enrichment score serves as an important quality control metric for ATACseq data. I want to write a script for single cell ATACseq data. From the Encode page: Transcription Start Site (TSS) Enrichment Score - The TSS enrichment calculation is a signal to noise calculation. The reads around a reference set of TSSs are collected to form an aggregate distribution of reads centered on the TSSs and extending to 1000 bp in either direction (for a total of 2000bp).

clustering scATACseq data: the TF-IDF way

scATACseq data are very sparse. It is sparser than scRNAseq. To do clustering of scATACseq data, there are some preprocessing steps need to be done. I want to reproduce what has been done after reading the method section of these two recent scATACseq paper: A Single-Cell Atlas of In Vivo Mammalian Chromatin Accessibility Darren Cell 2018 Latent Semantic Indexing Cluster Analysis In order to get an initial sense of the relationship between individual cells, we first broke the genome into 5kb windows and then scored each cell for any insertions in these windows, generating a large, sparse, binary matrix of 5kb windows by cells for each tissue.

Split a 10xscATAC bam file by cluster

I want to split the PBMC scATAC bam from 10x by cluster id. So, I can then make a bigwig for each cluster to visualize in IGV. The first thing I did was googling to see if anyone has written such a tool (Do not reinvent the wheels!). People have done that because I saw figures from the scATAC papers. I just could not find it. Maybe I need to refine my googling skills.

How to make a transcript to gene mapping file

I need a transcript to gene mapping file for Salmon. I am aware of annotation bioconductor packages that can do this job. However, I was working on a species which does not have the annotation in a package format (I am going to use Drosphila as an example for this blog post). I had to go and got the gtf file and made such a file from scratch. Please read the specifications of those two file formats.