single cell RNAseq Please read the following posts by Dave Tang. When I google, I always find his posts on top of the pages. Thanks for sharing your knowledge.
From the 10x manual:
The final Single Cell 3’ Libraries contain the P5 and P7 primers used in Illumina bridge amplification PCR. The 10x Barcode and Read 1 (primer site for sequencing read 1) is added to the molecules during the GEMRT incubation.