ScRNAseq

Run Rstudio server with singularity on HPC

Background Please read the following before go ahead: what is docker? what is Rocker? what is singularity? from Harvard Research computing website: Odyssey has singularity installed. Why Singularity? There are some important differences between Docker and Singularity: Docker and Singularity have their own container formats. Docker containers may be imported to run via Singularity. Docker containers need root privileges for full functionality which is not suitable for a shared HPC environment.

understand 10x scRNAseq and scATAC fastqs

single cell RNAseq Please read the following posts by Dave Tang. When I google, I always find his posts on top of the pages. Thanks for sharing your knowledge. https://davetang.org/muse/2018/06/06/10x-single-cell-bam-files/ https://davetang.org/muse/2018/08/09/getting-started-with-cell-ranger/ From the 10x manual: The final Single Cell 3’ Libraries contain the P5 and P7 primers used in Illumina bridge amplification PCR. The 10x Barcode and Read 1 (primer site for sequencing read 1) is added to the molecules during the GEMRT incubation.

Evaluating single cell RNAseq cluster stability

An R package for evaluating and visualizing scRNAseq cluster stability